Single-base mutations have an important role in cancer and other human diseases, and they can be useful markers for diagnostic tests. Mutations in viral genomes can cause resistance to antiviral drugs ...

Rolling-circle amplification (RCA) driven by DNA polymerase can replicate circularized oligonucleotide probes with either linear or geometric kinetics under isothermal conditions. In the presence of ...

Earlier this year, Applied Biosystems used the SOLiD 3 System’s underlying oligonucleotide ligation and detection technology to sequence a human genome for less than $60,000 [in 4 weeks]. Technical ...

October 27, 2007 At the 57th annual meeting of the American Society of Human Genetics, Applied Biosystems announced the worldwide commercial availability of SOLiD, the company’s next-generation DNA ...

It's a given that certain bacteria and viruses can cause illness and disease, but the real culprits are the sequences of concern that lie within the genomes of these microbes. Calling them out is ...

DNA diagnostics, the detection of specific DNA sequences, will play an increasingly important role in medicine as the molecular basis of human disease is defined. Here, we demonstrate an automated, ...

We have used a specific deoxyoligonucleotide probe to detect gastrin mRNA in poly(A)-enriched RNA preparations from hog antrum. The nucleotide sequence of the oligonucleotide, ...

Whole-genome sequencing may revolutionize medical diagnostics through rapid identification of alleles that cause disease. However, even in cases with simple patterns of inheritance and unambiguous ...

Applied Biosystems has announced the launch of the early-access program for its next-generation DNA sequencing system. The company said that it has shipped initial units of its SOLiD™ System to ...

In recent years, the landscape of sequencing technologies has undergone a transformative shift, transitioning from research settings to the realm of clinical laboratories. This transformation has been ...

Here are steps you can take to increase the odds of generating correct clones. PCR amplicons are commonly used for cloning. Primers made from synthetic oligonucleotides often contain a small ...

In the post-genome era, the next big challenge lies in mapping the proteome of every cell type, a daunting task indeed. One obstacle will be to identify trace amounts of proteins in cell or tissue ...